454 DNA Sequencing
Costs
Each library prep has an associated cost. The cost of the first prep is included in the run cost. Additional libraries (for DNA delivered at the same time) are $100 each. We assume that MID-tagged samples will be pooled prior to emPCR, otherwise the costs per sample increase $200-$800. Funds for the purchase of the 454 and associated instruments were provided by the Georgia Research Alliance. Some technical personnel are at least partially funded by the state of Georgia (through positions supported by UGA). The GGF believes it is important to support research throughout Georgia, thus, academic researchers from the state of Georgia will enjoy a significant cost savings.
Table 2. Costs for researchers from different organizations, assuming a single sample. The cost for additional libraries included in the same run as the first sample are also shown.
| Type of Organization | Costs | ||||
|---|---|---|---|---|---|
| Full run | Half-run | Quarter-run | Minimum | Libraries | |
| UGA | $10,750 | $6,650 | $4,000 | $1,000 | $100 |
| Georgia Academic | $10,750 | $6,650 | $4,000 | $1,000 | $100 |
| Non-Georgia Academic | $12,900 | $8,100 | $4,800 | $1,250 | $125 |
| Commercial | $16,125 | $10,125 | $6,000 | $1,750 | $175 |
Table 3. Example costs for a Georgia Academic Researcher that match the experiments described in Table 1 (minimum sequence expectations).
| Type of Library | Costs | |||
|---|---|---|---|---|
| Full run | Half-run | Quarter-run | Minimum | |
| Shotgun Library, single sample | $10,750 | $6,650 | $4,000 | $1,000 |
| cDNA, single sample | $10,750 | $6,650 | $4,000 | $1,000 |
| MID-tags, 8 samples | $11,450 | $7,350 | $4,700 | $1,700 |
| cDNA, 8 samples | $11,450 | $7,350 | $4,700 | $1,700 |
If at least half the expected number of reads are obtained, then researchers will be billed/invoiced proportionally (e.g., if 810,000 reads are obtained from a single shotgun library full run for a Georgia academic researcher, then [810,000/900,000] x $10,750 = 0.90 x $10,750 = $9,675).
